A NOVEL INDIRECT PATHWAY OF CRF INNERVATION TO PERIFORNICAL OREXIN NEURONS IS RELAYED THROUGH THE LATERAL SEPTUM

A Novel Indirect Pathway of CRF Innervation to Perifornical Orexin Neurons is Relayed Through the Lateral Septum Timothy D. Skog Director: Patrick J. Ronan, PhD Orexin (Orx) and corticotropin releasing factor (CRF) play integral, sometimes parallel, roles in a host of arousal/stress responses. These neuromodulators have been implicated in a variety of stress-induced psychiatric disorders including addiction and affective disorders. Previous work has indicated that these systems interact and regulate each other—perhaps providing a feed-forward mechanism for enhancing and fine-tuning stress responses. This is implied by the fact that orexinergic neurons in the hypothalamus receive innervation from many CRF-rich neuronal fields including the paraventricular nucleus of the hypothalamus (PVN), bed nucleus of the stria terminalis (BNST) and central nucleus of the amygdala (CeA). We sought to clarify if these CRF afferent pathways include CRF neurons and to determine whether these inputs have any topographical organization. To accomplish this, we used a combination of neuronal tracing methods and immunohistochemistry to visualize the distribution and anatomy of these systems in various brain regions. We provide further evidence that CRF neurons in these regions specifically project to Orx neuron fields, as well as describe a novel circuit pathway for indirect CRF innervation of orexinergic neurons in the hypothalamus through the lateral septum. There appears to be specific topographic distribution of inputs supporting the hypothesis that perifornical Orx neurons preferentially contribute to stress and anxiety responses

Forest Carbon Sequestration under the U.S. Biofuel Energy Policies

This paper analyzes impacts of the U.S. biofuel energy policies on the carbon sequestration by forest products, which is expressed as Harvested Wood Products (HWP) Contribution under the United Nations Framework Convention on Climate Change. Estimation for HWP Contribution is based on tracking carbon stock stored in wood and paper products in use and in solid-waste disposal sites (SWDS) from domestic consumption, harvests, imports, and exports. For this analysis, we hypothesize four alternative scenarios using the existing and pending U.S. energy policies by requirements for the share of biofuel to total energy consumption, and solve partial equilibrium for the U.S. timber market by 2030 for each scenario. The U.S. Forest Products Module (USFPM), created by USDA Forest Service Lab, operating within the Global Forest Products Model (GFPM) is utilized for projecting productions, supplies, and trade quantities for the U.S. timber market equilibrium. Based on those timber market components, we estimate scenario-specific HWP Contributions under the Production, the Stock Change, and the Atmospheric Approach suggested by Intergovernmental Panel on Climate Change (IPCC) Guidelines for National Greenhouse Gas Inventories using WOODCARB II created by VTT Technical Research Centre of Finland and modified by USDA Forest Service Lab. Lastly, we compare estimated results across alternative scenarios. Results show that HWP Contributions for the baseline scenario in 2009 for all approaches are estimated higher than estimates reported by U.S. Environmental Protection Agency in 2011, (e.g., 22.64 Tg C/ year vs 14.80 Tg C/ year under the Production Approach), which is due to the economic recovery, especially in housing construction, assumed in USFPM/GFPM. Projected HWP Contribution estimates show that the Stock Change Approach, which used to provide the highest estimates before 2009, estimate HWP Contribution lowest after 2009 due to the declining annual net imports. Though fuel wood consumption is projected to be expanded as an alternative scenario requires higher wood fuel share to total energy consumption, the overall impacts on the expansion in other timber products are very modest across scenarios in USFPM/GFPM. Those negligible impacts lead to small differences of HWP Contribution estimates under all approaches across alternative scenarios. This is explained by the points that increasing logging residues are more crucial for expansion in fuel wood projections rather than the expansion of forest sector itself, and that the current HWP Contribution does not include carbon held in fuel wood products by its definition.Forest Products, Carbon Sequestration, Biofuel Policies, HWP Contribution, Resource /Energy Economics and Policy,

Review of Person Re-identification Techniques

Person re-identification across different surveillance cameras with disjoint fields of view has become one of the most interesting and challenging subjects in the area of intelligent video surveillance. Although several methods have been developed and proposed, certain limitations and unresolved issues remain. In all of the existing re-identification approaches, feature vectors are extracted from segmented still images or video frames. Different similarity or dissimilarity measures have been applied to these vectors. Some methods have used simple constant metrics, whereas others have utilised models to obtain optimised metrics. Some have created models based on local colour or texture information, and others have built models based on the gait of people. In general, the main objective of all these approaches is to achieve a higher-accuracy rate and lowercomputational costs. This study summarises several developments in recent literature and discusses the various available methods used in person re-identification. Specifically, their advantages and disadvantages are mentioned and compared.Comment: Published 201

Conceptual and Empirical Themes regarding the Design of Technology Transfer Programs: A Review of Wood Utilization Research in the United States

Transfer of technologies produced by research is critical to innovation within all organizations. The intent of this paper is to take stock of the conceptual underpinnings of technology transfer processes as they relate to wood utilization research and to identify conditions that promote the successful transfer of research results. Conceptually, research utilization can be viewed from multiple perspectives, including the haphazard diffusion of knowledge in response to vague and imprecise demands for information, scanning of multiple information sources by individuals and organizations searching for useful scientific knowledge, engagement of third parties to organize research results and communicate them to potential users, and ongoing and active collaboration between researchers and potential users of research. Empirical evidence suggests that various types of programs can promote technology transfer (venture capital, angel investors, business incubators, extension services, tax incentives, and in-house entities), the fundamental effectiveness of which depends on research results that are scientifically valid and consistent with the information needs of potential users. Furthermore, evidence suggests preference toward programs that are appropriately organized and governed, suitably led and creatively administered, and periodically evaluated in accordance with clear standards of success

Prostate cancer-derived urine exosomes: a novel approach to biomarkers for prostate cancer

Herein, we describe a novel approach in the search for prostate cancer biomarkers, which relies on the transcriptome within tumour exosomes. As a proof-of-concept, we show the presence of two known prostate cancer biomarkers, PCA-3 and TMPRSS2:ERG the in exosomes isolated from urine of patients, showing the potential for diagnosis and monitoring cancer patients status

Determining the best population-level alcohol consumption model and its impact on estimates of alcohol-attributable harms

BACKGROUND: The goals of our study are to determine the most appropriate model for alcohol consumption as an exposure for burden of disease, to analyze the effect of the chosen alcohol consumption distribution on the estimation of the alcohol Population- Attributable Fractions (PAFs), and to characterize the chosen alcohol consumption distribution by exploring if there is a global relationship within the distribution. METHODS: To identify the best model, the Log-Normal, Gamma, and Weibull prevalence distributions were examined using data from 41 surveys from Gender, Alcohol and Culture: An International Study (GENACIS) and from the European Comparative Alcohol Study. To assess the effect of these distributions on the estimated alcohol PAFs, we calculated the alcohol PAF for diabetes, breast cancer, and pancreatitis using the three above-named distributions and using the more traditional approach based on categories. The relationship between the mean and the standard deviation from the Gamma distribution was estimated using data from 851 datasets for 66 countries from GENACIS and from the STEPwise approach to Surveillance from the World Health Organization. RESULTS: The Log-Normal distribution provided a poor fit for the survey data, with Gamma and Weibull distributions providing better fits. Additionally, our analyses showed that there were no marked differences for the alcohol PAF estimates based on the Gamma or Weibull distributions compared to PAFs based on categorical alcohol consumption estimates. The standard deviation of the alcohol distribution was highly dependent on the mean, with a unit increase in alcohol consumption associated with a unit increase in the mean of 1.258 (95% CI: 1.223 to 1.293) (R2 = 0.9207) for women and 1.171 (95% CI: 1.144 to 1.197) (R2 = 0. 9474) for men. CONCLUSIONS: Although the Gamma distribution and the Weibull distribution provided similar results, the Gamma distribution is recommended to model alcohol consumption from population surveys due to its fit, flexibility, and the ease with which it can be modified. The results showed that a large degree of variance of the standard deviation of the alcohol consumption Gamma distribution was explained by the mean alcohol consumption, allowing for alcohol consumption to be modeled through a Gamma distribution using only average consumption

Microguards and micromessengers of the genome

The regulation of gene expression is of fundamental importance to maintain organismal function and integrity and requires a multifaceted and highly ordered sequence of events. The cyclic nature of gene expression is known as ‘transcription dynamics’. Disruption or perturbation of these dynamics can result in significant fitness costs arising from genome instability, accelerated ageing and disease. We review recent research that supports the idea that an important new role for small RNAs, particularly microRNAs (miRNAs), is in protecting the genome against short-term transcriptional fluctuations, in a process we term ‘microguarding’. An additional emerging role for miRNAs is as ‘micromessengers’—through alteration of gene expression in target cells to which they are trafficked within microvesicles. We describe the scant but emerging evidence that miRNAs can be moved between different cells, individuals and even species, to exert biologically significant responses. With these two new roles, miRNAs have the potential to protect against deleterious gene expression variation from perturbation and to themselves perturb the expression of genes in target cells. These interactions between cells will frequently be subject to conflicts of interest when they occur between unrelated cells that lack a coincidence of fitness interests. Hence, there is the potential for miRNAs to represent both a means to resolve conflicts of interest, as well as instigate them. We conclude by exploring this conflict hypothesis, by describing some of the initial evidence consistent with it and proposing new ideas for future research into this exciting topic

Microparticle-mediated transfer of the viral receptors CAR and CD46, and the CFTR channel in a CHO cell model confers new functions to target cells

Cell microparticles (MPs) released in the extracellular milieu can embark plasma membrane and intracellular components which are specific of their cellular origin, and transfer them to target cells. The MP-mediated, cell-to-cell transfer of three human membrane glycoproteins of different degrees of complexity was investigated in the present study, using a CHO cell model system. We first tested the delivery of CAR and CD46, two monospanins which act as adenovirus receptors, to target CHO cells. CHO cells lack CAR and CD46, high affinity receptors for human adenovirus serotype 5 (HAdV5), and serotype 35 (HAdV35), respectively. We found that MPs derived from CHO cells (MP-donor cells) constitutively expressing CAR (MP-CAR) or CD46 (MP-CD46) were able to transfer CAR and CD46 to target CHO cells, and conferred selective permissiveness to HAdV5 and HAdV35. In addition, target CHO cells incubated with MP-CD46 acquired the CD46-associated function in complement regulation. We also explored the MP-mediated delivery of a dodecaspanin membrane glycoprotein, the CFTR to target CHO cells. CFTR functions as a chloride channel in human cells and is implicated in the genetic disease cystic fibrosis. Target CHO cells incubated with MPs produced by CHO cells constitutively expressing GFP-tagged CFTR (MP-GFP-CFTR) were found to gain a new cellular function, the chloride channel activity associated to CFTR. Time-course analysis of the appearance of GFP-CFTR in target cells suggested that MPs could achieve the delivery of CFTR to target cells via two mechanisms: the transfer of mature, membrane-inserted CFTR glycoprotein, and the transfer of CFTR-encoding mRNA. These results confirmed that cell-derived MPs represent a new class of promising therapeutic vehicles for the delivery of bioactive macromolecules, proteins or mRNAs, the latter exerting the desired therapeutic effect in target cells via de novo synthesis of their encoded proteins